Integrative Modeling for Constructing High-Resolution
Atomistic Models of Nucleosomes
National Center for Biotechnology Information
National Library of Medicine
National Institutes of Health
Wed, November 14, 2018 - 4:00 PM
Karl Herzfeld Auditorium of Hannan Hall - Rm 108
The Nucleosomes are basic units of chromatin and their variability is important for epigenetic regulation of transcription and replication; it can be modulated, in turn, via conformational changes of histone core and of nucleosomal DNA, by post-translational modifications and by using different histone variants. I will talk about in silico and in vitro DNA footprinting experiments to explore conformational polymorphism of nucleosomes both at local and global scales. Such integrative studies enabled us to get insights into the functionally relevant rearrangements in
nucleosomes including the coupling between the conformations of histone tails and the DNA geometry, the formation of twist defects within DNA and the rearrangement of histone–DNA interactions. In addition, our methods produced high resolution atomistic structural models of different variant nucleosomes verified and refined by using experimental data.
Refreshments served at 3:45 PM
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